(Fertil Steril (R) 2011; 96: 974-9 (C)2011 by American Society f

(Fertil Steril (R) 2011; 96: 974-9. (C)2011 by American Society for Reproductive Medicine.)”
“Different definitions of response, remission, and recovery are used in schizophrenia research, which makes comparing and applying results in clinical practice difficult. Response criteria are often based on reductions in rating scale scores (eg, >= 20% reduction from baseline). However, when reduction scores from rating scales, such as the Positive and Negative Syndrome Scale (PANSS) and Brief Psychiatric Rating Scale (BPRS), are linked to Clinical Global Impressions (CGI) scores, which are more easily understood, HDAC inhibitor rating scale

scores have better clinical application. This linking process also reveals that the widely used response cutoff of 20% does not reflect clinically meaningful improvement in patients with acute, nonrefractory schizophrenia. This article provides suggestions for selecting response criteria, displaying responder rates, and using standard definitions (eg, remission, recovery) in research studies. The ultimate goal of recovery in

schizophrenia treatment includes sustained symptom resolution and a return to full functioning.”
“Background: Spasticity is a disabling complication of multiple sclerosis, affecting many patients with the condition. We report the first Phase 3 placebo-controlled study of an oral antispasticity agent to use an enriched study this website design.\n\nMethods: A 19-week follow-up, multicentre, double-blind, randomized, placebo-controlled, parallel-group study in subjects with multiple sclerosis spasticity not fully relieved with current antispasticity therapy. Subjects were treated with nabiximols, as add-on therapy, in a single-blind manner for 4 weeks, after which those achieving an improvement in spasticity of >= 20% progressed to a 12-week randomized, placebo-controlled phase.\n\nResults: Of the 572 subjects enrolled, 272 achieved a >= Stem Cells & Wnt inhibitor 20% improvement after 4 weeks of single-blind treatment, and 241 were randomized. The primary end-point was the difference between treatments in the mean spasticity Numeric Rating Scale (NRS)

in the randomized, controlled phase of the study. Intention-to-treat (ITT) analysis showed a highly significant difference in favour of nabiximols (P = 0.0002). Secondary end-points of responder analysis, Spasm Frequency Score, Sleep Disturbance NRS Patient, Carer and Clinician Global Impression of Change were all significant in favour of nabiximols.\n\nConclusions: The enriched study design provides a method of determining the efficacy and safety of nabiximols in a way that more closely reflects proposed clinical practice, by limiting exposure to those patients who are likely to benefit from it. Hence, the difference between active and placebo should be a reflection of efficacy and safety in the population intended for treatment.

“Fluorescence and

electron paramagnetic resonance

“Fluorescence and

electron paramagnetic resonance measurements were used to study selenium influence on photosystem activity in rape seedlings affected by Cd stress. Water cultures containing Hoagland nutrients were supplemented with 400 mu M of CdCl(2), 2 mu M of Na(2)SeO(4) and a mixture of both CdCl(2) and Na(2)SeO(4). The seedlings were cultured till the first leaf reached about 1 cm in length. Cadmium-induced changes in the AZD0530 in vitro activity of both photosystems were partly diminished by Se presence in the nutrient medium. Electron microscopy photographs confirmed less degradation in chloroplasts of plants cultured on media containing Se. It is suggested that sucrose groups of starch, which is deposited in greater amounts in Cd-stressed plants, may act as traps for free radicals produced under those conditions.”
“The ubiquitous human polyomavirus JC virus (JCV) is the established etiological agent of the debilitating and often fatal demyelinating disease, progressive multifocal leukoencephalopathy (PML). Most healthy individuals

have been infected with JCV and generate an selleck inhibitor immune response to the virus, yet remain persistently infected at subclinical levels. The onset of PML is rare in the general population, but has become an increasing concern in immunocompromised patients, where reactivation of JCV leads to uncontrolled replication in the CNS. Understanding viral persistence and the normal immune response to JCV provides insight into the circumstances

which could lead to viral resurgence. Further, clues on the potential mechanisms of reactivation may be gleaned from the crosstalk among JCV and HIV-1, as well as the impact of monoclonal antibody therapies used for the treatment of autoimmune disorders, including multiple sclerosis, on the development of PML. In this review, we will discuss what is known about viral persistence and the immune response to JCV replication in immunocompromised individuals to elucidate the deficiencies in viral containment that permit viral reactivation and spread.”
“Both ligand-dependent and ligand-independent activation of estrogen receptor (ER) is modulated by receptor phosphorylation and results in activation of the ER-dependent pathways that are involved in endometrioid endometrial cancer (EEC) pathogenesis. It is also known that the mammalian target of rapamycin learn more (mTOR)/p70 S6 kinase 1 (S6K1) and MAPK/p90 ribosomal S6 kinase (RSK) signaling pathways coordinately regulate phosphorylated-ER at Ser(167) (p-Ser(167)-ER). However, the expression of p-Ser(167)-ER in EEC and its prognostic role in ECC is largely unexplored. The purpose of the present study was to investigate the expression of p-Ser(167)-ER in ECC and its relationship with prognosis. Immunohistochemical staining of primary EEC surgical specimens (n=103) was carried out using antibodies specific for p-Ser(167)-ER and for p-mTOR/p-S6K1 and p-MAPK/p-RSK.

We demonstrated, by all methods used, that both mutant proteins r

We demonstrated, by all methods used, that both mutant proteins reduced SRY DNA binding activity. The three-dimensional structure of SRY suggested that besides the HMG box, the carboxy-terminal

region of SRY interacts with DNA.\n\nIn conclusion, we identified two SRY mutations and a polymorphism in two patients with 46,XY complete PGD, demonstrating the importance of the carboxy-terminal region of SRY in DNA Evofosfamide manufacturer binding activity. (c) 2008 Elsevier Ireland Ltd. All rights reserved.”
“Lymphangioma usually occurs in children and usually involves the skin. Mesenteric lymphangioma is extremely rare in adults. Typically, lymphangioma appears on computed tomography (CT) as a lower attenuation of a cystic mass, however, some cases appear to be a solid mass. We describe the CT and 18F-FDG positron emission tomography/CT appearance in a case of jejunal and mesenteric cavernous lymphangiomatosis mimicking metastasis in an adult patient with rectal cancer. check details (c) 2009 The WJG Press and Baishideng. All rights reserved.”
“Details of the modern history of paediatric surgery in the United Kingdom with particular emphasis on the 3 main training centres in England in the 1960s to 1970s are discussed. The genesis of the National Health

Service and of the British Association of Paediatric Surgeons and their influence on the establishment and siting of regional centres, education and training, and centralization of rare conditions is highlighted. (C) 2012 Elsevier Inc. All rights reserved.”
“Erythrocytes evolved to carry out

the crucial function of transportation of oxygen from lungs to various tissues in the body and to carry back carbon dioxide. Absence of a nucleus in erythrocytes facilitates their easy movement in blood capillaries and make space for large quantities of haemoglobin, the oxygen carrier molecule, but renders the cells ill equipped to effectively repair themselves from oxidative and other buy Quisinostat damages. Turnover rate of erythrocytes is fast and the average life span of murine erythrocytes in blood is only 50 days. What age related changes occur in circulating erythrocytes and which of these changes are crucial determining factors in triggering the destruction of old erythrocytes, are not well understood. The main reason for a lack of information in this area is the fact that there were not available any good and easy to perform technique to identify circulating erythrocytes of different age groups. We have recently developed a technique that involved in vivo biotinylation of erythrocytes in two steps that has allowed us for the first time to objectively assess age-related changes in blood erythrocytes. We have utilized this technique to study various aspects of aging of murine erythrocytes. On the basis of these results, our current thinking about the process of erythrocyte aging in vivo is summarized in the this article.

“Oxidative stress has been suggested as a potential contri

“Oxidative stress has been suggested as a potential contributor to the development of diabetic complications. In this study, we investigated the protective effect of a strong antioxidant copper complex against streptozotocin (STZ)-induced diabetes in animals. Out of four copper complexes used, copper(II) (3,5-diisopropyl salicylate)(4) (Cu(II)DIPS) was found to be the most potent antioxidant-copper complex. Pretreatment with Cu(II)DIPS (5 mg/kg) twice a week prior to the injection of streptozotocin (50 mg/kg) has

reduced the level of hyperglycemia by 34 % and the mortality rate by 29 %. Injection of PI3K Inhibitor Library research buy the same dosage of the ligand 3,5-diisopropyl salicylate has no effect on streptozotocin-induced VX-770 purchase hyperglycemia. The same copper complex has neither hypoglycemic activity when injected in normal rats nor antidiabetic activity when injected in STZ-induced diabetic rats. The protective effect of Cu(II)DIPS could be related to its strong antioxidant activity compared to other copper complexes median effective concentration (MEC) = 23.84 mu g/ml and to Trolox MEC = 29.30 mu g/ml. In addition, it reduced serum 8-hydroxy-2′-deoxyguanosine, a biomarker of oxidative DNA damage, by

29 %. This effect may explain why it was not effective against diabetic rats, when beta Langerhans cells were already destroyed. Similar protective activities were reported by other antioxidants like Trolox.”
“It is possible to achieve substantial initial control of systemic vasculitis in

the majority of patients. However the ‘target’ has shifted considerably AZD5582 over the last 20-30 years from keeping patients alive to maintaining good quality disease control, avoiding the development of comorbidities either as a result of disease or treatment, and also preventing relapses. This expansion of potential targets that can be achieved in systemic vasculitis has arisen because we have more effective therapies, but more importantly we have developed a framework within which targets can be created reproducibly. In other words we have much clearer definitions of what constitutes clinical disease activity, relapse, remission and morbidity. These targets are based on simple clinical evaluation, limited laboratory assessments of patients that can be undertaken by any secondary care facility. As a result of this they remain at a clinical level and may not address the most important targets, which are curing disease and that would be the aspiration to move towards. The first step towards that is to move from clinically-based targets towards mechanistic targets based primarily around the pathophysiological drivers of disease. That in turn may lead to identification of specific targets that can turn off disease.

In addition, we have obtained a new crystal structure of the RAGE

In addition, we have obtained a new crystal structure of the RAGE VC1 fragment. The packing in both crystal structures reveals an association of the RAGE molecules through contacts between two V domains and the physiological relevance of this homodimerization mode is discussed. Based on homology with single-pass

transmembrane receptors, we also suggest RAGE dimerization through a conserved GxxxG motif within its transmembrane domain. A multimodal homodimerization strategy of RAGE is proposed to form the structural basis for ligand-specific complex formation and signalling functions, as well as for RAGE-mediated cell adhesion. Structured digital abstract hRAGE_VC1C2 and hRAGE_VC1C2 bind by x-ray crystallography (View interaction) CHIR98014 hRAGE_VC1 and hRAGE_VC1 bind by x-ray crystallography (View interaction)”
“Glutamate dehydrogenase (GDH) is a crucial enzyme on selleck the crossroads of amino acid and energy metabolism and it is operating in all domains of life. According to current knowledge GDH is present only in one functional isoform in most animals, including mice. In addition to this housekeeping enzyme (hGDH1 in humans), humans and apes have acquired a second isoform (hGDH2) with a distinct tissue expression profile. In the current study we have cloned both mouse and human GDH constructs containing

FLAG and (His)(6) small genetically-encoded tags, respectively. The hGDH1 and hGDH2 constructs containing N-terminal (His)(6) tags were successfully

expressed in Sf9 cells and the recombinant proteins were isolated to a parts per thousand yen95 https://www.selleckchem.com/products/jnk-in-8.html % purity in a two-step procedure involving ammonium sulfate precipitation and Ni2+-based immobilized metal ion affinity chromatography. To explore whether the presence of the FLAG and (His)(6) tags affects the cellular localization and functionality of the GDH isoforms, we studied the subcellular distribution of the expressed enzymes as well as their regulation by adenosine diphosphate monopotassium salt (ADP) and guanosine-5′-triphosphate sodium salt (GTP). Through immunoblot analysis of the mitochondrial and cytosolic fraction of the HEK cells expressing the recombinant proteins we found that neither FLAG nor (His)(6) tag disturbs the mitochondrial localization of GDH. The addition of the small tags to the N-terminus of the mature mitochondrial mouse GDH1 or human hGDH1 and hGDH2 did not change the ADP activation or GTP inhibition pattern of the proteins as compared to their untagged counterparts. However, the addition of FLAG tag to the C-terminus of the mouse GDH left the recombinant protein fivefold less sensitive to ADP activation. This finding highlights the necessity of the functional characterization of recombinant proteins containing even the smallest available tags.”
“Currently, one of the biggest challenges faced by organic no-tillage farming is weed control. Thus, the use of cropping practices that help in the control of weeds is extremely important.

85 (0 80-0 90 I-2 96%), respectively Regarding periprocedural ou

85 (0.80-0.90 I-2 96%), respectively. Regarding periprocedural outcomes, TF reduced risk of bleedings and strokes (OR of 0.74 [0.66-0.82 I-2 95%] and 0.91 [0.83-0.99] I-2 86%, respectively). Conclusions: The TF approach reduces mortality in TAVI patients, due to lower rates of periprocedural bleedings

and strokes.”
“Mental illness affects the lives of a significant number of Australians. In addition to pharmacological and psychological interventions, exercise has demonstrated benefits for people with mental illness including symptom reduction, improved cardiovascular risk profile and improved physical capacity. Unfortunately, evidence shows that clinician-delivered exercise advice is not routinely offered. This is despite patient acceptability for exercise. This article summarises the recent evidence supporting the prescription of exercise for people with buy 4SC-202 mental illness and offers a model incorporating basic exercise prescription, and referral pathways for specialised advice. Current exercise prescription patterns for people with mental illness may not meet patient expectations; therefore, clinicians should consider

exercise referral schemes to increase the accessibility of interventions for people with a mental illness.”
“Podolin PL, Foley JP, Carpenter JNJ-26481585 manufacturer DC, Bolognese BJ, Logan GA, Long E 3rd, Harrison OJ, Walsh PT. T cell depletion protects against alveolar destruction due to chronic cigarette smoke exposure in mice. Am J Physiol Lung Cell Mol Physiol 304: L312-L323, 2013. First published January 4, 2013; doi:10.1152/ajplung.00152.2012.-The role of T cells in chronic obstructive pulmonary disease (COPD) is not well understood. We have previously demonstrated that chronic cigarette smoke

exposure can lead to the accumulation of CD4(+) and CD8(+) T cells in the alveolar airspaces in a mouse model of COPD, implicating these cells in disease pathogenesis. However, whether specific inhibition of T cell responses represents a therapeutic strategy has not been fully investigated. In this study inhibition of T cell responses through specific depleting antibodies, or the T cell immunosuppressant see more drug cyclosporin A, prevented airspace enlargement and neutrophil infiltration in a mouse model of chronic cigarette smoke exposure. Furthermore, individual inhibition of either CD4(+) T helper or CD8(+) T cytotoxic cells prevented airspace enlargement to a similar degree, implicating both T cell subsets as critical mediators of the adaptive immune response induced by cigarette smoke exposure. Importantly, T cell depletion resulted in significantly decreased levels of the Th17-associated cytokine IL-17A, and of caspase 3 and caspase 7 gene expression and activity, induced by cigarette smoke exposure. Finally, inhibition of T cell responses in a therapeutic manner also inhibited cigarette smoke-induced airspace enlargement, IL-17A expression, and neutrophil influx in mice.

While previous studies have shown that Appl1 plays a pivotal role

While previous studies have shown that Appl1 plays a pivotal role in adiponectin signaling and insulin secretion, the physiological functions Selleck KPT-8602 of Appl2 are largely unknown. Results: In the present study, the role of Appl2 in sepsis shock was investigated by using Appl2 knockout (KO) mice. When challenged with lipopolysaccharides (LPS), Appl2 KO mice exhibited more severe symptoms of endotoxin shock,

accompanied by increased production of proinflammatory cytokines. In comparison with the wild-type control, deletion of Appl2 led to higher levels of TNF-alpha and IL-1 beta in primary macrophages. In addition, phosphorylation of Akt and its downstream effector NF-kappa B was significantly enhanced. By co-immunoprecipitation, p53 inhibitor we found that

Appl2 and Appl1 interacted with each other and formed a complex with PI3K regulatory subunit p85 alpha, which is an upstream regulator of Akt. Consistent with these results, deletion of Appl1 in macrophages exhibited characteristics of reduced Akt activation and decreased the production of TNF alpha and IL-1 beta when challenged by LPS. Conclusions: Results of the present study demonstrated that Appl2 is a critical negative regulator of innate immune response via inhibition of PI3K/Akt/NF-kappa B signaling pathway by forming a complex with Appl1 and PI3K.”
“Phenylketonuria (PKU) is caused by a defect in phenylalanine hydroxylase (PAH). More than 500 mutations have been reported for the gene encoding PAH. However, approximately 1%-5% of these include large deletions and large duplications that cannot be detected by conventional methods. In this

report we tried to fully characterize a PAH-deficient patient. The patient was a 2-year-old Japanese boy who was diagnosed with classical PKU at the time of neonatal screening, which was confirmed by the tetrahydrobiopterin-loading GSK1838705A test. PCR-related direct sequencing and multiplex ligation-dependent probe amplification (MLPA) were used to analyze of the PAH of the patient. Using PCR-related direct sequencing method, we could detect only a heterozygous novel missense mutation: p.136G bigger than C (p.G46R). A second mutation was detected by MLPA. The patient was heterozygous for a novel large deletion of exons 12 and 13: c.1200-?_1359+?del (EX12_13del). For genetic counseling, an accurate genetic diagnosis is often necessary. Through a combination of MLPA and conventional methods, the success rate of PAH mutation identification can be close to 100%.”
“The fast-start escape response is critically important to avoid predation, and axial movements driving it have been studied intensively. Large median dorsal and anal fins located near the tail have been hypothesized to increase acceleration away from the threat, yet the contribution of flexible median fins remains undescribed.

We included 95 normal controls in the study DNA from peripheral

We included 95 normal controls in the study. DNA from peripheral blood was used in genotyping for the FBN1 R2726W mutation using pyrosequencing.\n\nResults: No mutant alleles were identified in any patients or controls.\n\nConclusions: selleck chemicals llc The R2726W mutation is not responsible for skeletal malformation of primary protrusio acetabuli in our population, although there may be unidentified genetic variants in either FBN1 or other genes that control acetabular morphology.”

thrombin-binding aptamer (TBA) is a consensus DNA 15-mer that binds specifically to human alpha-thrombin at nanomolar concentrations and inhibits its procoagulant functions. Recently, a modified TBA (mTBA) containing a 5′-5′ inversion-of-polarity site has been shown to be more stable and to possess a higher thrombin affinity than its unmodified counterpart. The structure of the thrombin-TBA complex has previously been determined at

low resolution, but did not provide a detailed picture of the aptamer conformation or of the protein-DNA assembly, while that of the complex with mTBA is unknown. Crystallographic analysis of the thrombin-mTBA complex has been attempted. The crystals diffracted to 2.15 A resolution and belonged to space group LXH254 I222.”
“Objectives: We explored comfort levels of third-year medical (M3) students through two health behavior counseling (NBC) interactions with Standardized Patient Instructors (SPIs) in tobacco cessation (TCC) and nutrition

and physical activity (NPA).\n\nMethods: Nearly 200 M3s participated in two SPI HBC interactions; including a role-play interview and subsequent feedback session on performance. Students completed a 5-point Likert scale evaluation measuring pre- and post-comfort level on two HBC sessions.\n\nResults: Both interactions resulted in statistically significant increases in student’s pre- and post-interaction comfort levels. A paired-sample t-test revealed selleck chemicals a mean increase of 0.91 for TCC (t = 14.01, df = 197, p < 0.001), and a mean increase of 0.69 for NPA (t = 12.65, df = 198, p < 0.001).\n\nConclusion: The use of SPIs is a viable approach to exposing medical students and future doctors to health behavior counseling, and increasing comfort level with such skills. The SPI experience ensures that NBC opportunities are available and contain meaningful feedback on performance.\n\nPractice implications: Encouraging patient behavior modification is a skill that can be developed during undergraduate medical training. Combining HBC with SPI sessions and traditional learning approaches could prove effective in a curriculum intended to teach students strategies that improve patient health behavior. Published by Elsevier Ireland Ltd.”
“Objective: To describe a case of postmenopausal hyperandrogenemia in which a small ovarian tumor was ruled out by a positive metformin suppression test.

Prevention of obesity using various comprehensive programmes appe

Prevention of obesity using various comprehensive programmes appears to be very promising, although we must admit that several interventions had generally disappointing results compared with the objectives

and target initially fixed. Holistic programmes including nutritional education combined with promotion of physical activity and behaviour modification constitute the key factors in the prevention of childhood and adolescent obesity. The purpose of this programme was to incorporate nutrition/physical education as well as psychological aspects in selected secondary schools (9th grade, 14-17 years).\n\nMethods: The educational strategy was based on Cyclopamine inhibitor the development of a series of 13 practical workshops covering wide areas such as physical inactivity, body composition, sugar, energy density, invisible lipids, how to read food labels, is meal duration important? Do you eat with pleasure or not? Do you eat because you are hungry? Emotional eating. For teachers continuing education, a basic highly illustrated guide was developed as a companion booklet to the workshops. These materials were first validated by biology, physical education, dietician and psychologist teachers as well as school medical officers.\n\nResults: BI 2536 manufacturer Teachers considered the practical educational materials

innovative and useful, motivational and easy to understand. Up to now ( early 2008), the programme has been implemented in 50 classes or more from schools originating from three areas in the French part of Switzerland.

Based on the 1-week pedometer value assessed before and after the 1 school-year programme, an initial evaluation indicated that overall physical placidity was significantly decreased as evidenced by a significant Z-IETD-FMK cell line rise in the number of steps per day.\n\nConclusion: Future evaluation will provide more information on the effectiveness of the ADOS programme.”
“Introduction: Estrogen receptor (ER) beta is predicted to play an important role in prevention of breast cancer development and metastasis. We have shown previously that ER beta inhibits hypoxia inducible factor (HIF)-1 alpha mediated transcription, but the mechanism by which ER beta works to exert this effect is not understood.\n\nMethods: Vascular endothelial growth factor (VEGF) was measured in conditioned medium by enzyme-linked immunosorbent assays. Reverse transcription polymerase chain reaction (RT-PCR), Western blotting, immunoprecipitation, luciferase assays and chromatin immunoprecipitation (ChIP) assays were used to ascertain the implication of ER beta on HIF-1 function.\n\nResults: In this study, we found that the inhibition of HIF-1 activity by ER beta expression was correlated with ER beta’s ability to degrade aryl hydrocarbon receptor nuclear translocator (ARNT) via ubiquitination processes leading to the reduction of active HIF-1 alpha/ARNT complexes.

The pathogenesis of leukoaraiosis is

The pathogenesis of leukoaraiosis is Selleck GKT137831 poorly understood, even if chronic ischemia with consequent arteriolosclerosis probably due to endothelial dysfunction has been suggested. To date, treatment focuses only on prevention of lesion formation and progression by aggressive control of risk factors, beginning at an early age and continuing throughout life.\n\nL-Arginine, a semi-essential amino acid, is a precursor of NO in the reaction catalyzed by endothelial nitric oxide synthase and, it has been recently found to importantly influence endothelial function. Arginine supplementation has been demonstrated to be safe and effective therapy for many health conditions, particularly vascular diseases such as intermittent

claudication, angina pectoris, erectile dysfunction and MELAS.\n\nThus we hypothesize that, since a lack of endothelium-derived NO may be responsible for several features of LA, long-term administration of high oral doses of L-Arg may slow LA progression and the associated functional impairment. (C) 2011 Elsevier Ltd. All rights reserved.”
“Solid oxide fuel cells (SOFC) are

selleck the cleanest, most efficient, and cost-effective option for direct conversion to electricity of a wide variety of fuels. While significant progress has been made in anode materials with enhanced tolerance to coking and contaminant poisoning, cathodic polarization still contributes considerably to energy loss, more so at lower operating temperatures. Here we report a synergistic effect of co-doping in a cation-ordered double-perovskite material, PrBa0.5Sr0.5Co2-xFexO5+delta, which has created pore channels that dramatically enhance Adavosertib price oxygen ion diffusion and surface oxygen exchange while maintaining excellent compatibility and stability under operating conditions. Test cells based on these cathode materials demonstrate peak power densities similar to 2.2 W cm(-2) at 600

degrees C, representing an important step toward commercially viable SOFC technologies.”
“Interactions of the ESCRT complexes are critical for endosomal trafficking. We identify two domains with potential significance for this process. The MABP domain present in metazoan ESCRT-I/MVB12 subunits, Crag, a regulator of protein sorting, and bacterial pore-forming proteins might mediate novel membrane interactions in trafficking. The UBAP1-MVB12-associated UMA domain found in MVB12 and UBAP1 defines a novel adaptor that might recruit diverse targets to ESCRT-I.”
“Obesity-associated severe asthma is a distinct phenotype characterised by resistance to standard asthma therapies. Bariatric surgery appears to be a viable alternative for those who have failed trials of traditional weight loss methods. However, anaesthetic and surgical risks are potential barriers. We describe three patients with treatment-resistant obesity-associated severe asthma who underwent bariatric surgery without complications due to the multidisciplinary perioperative planning and care involved in these complex cases.