Internal quality control samples (25, 250 and 400 µg/l, respectively) prepared in analyte-free pooled human plasma were analysed with each sample batch and between every 10 sample selleck kinase inhibitor extracts. Samples with plasma quetiapine markedly (approximately 10%) above the calibration range were diluted in analyte-free human plasma and re-analysed. The limit of accurate measurement was 5 µg/l (200 µl sample). For both methods intra-assay precision (relative standard deviation, %) at analyte

concentrations of 25, 250 and 400 µg/l was 3–5% and the Inhibitors,research,lifescience,medical corresponding inter-assay precision was 2–5%. Statistical analysis Descriptive and statistical analyses were performed

using Microsoft Excel 2003. Quetiapine dose and plasma concentration were grouped Inhibitors,research,lifescience,medical as appropriate to facilitate data analysis. Results There were 946 samples from 487 patients (257 [53%] males, age at time of first sample [median (range)] 34 (14–87) years, and 230 (47%) females, aged 38 (10–92) years). Of these, 17 samples (6 males, age at time of first sample [median (range)] 17 (14–17) years, Inhibitors,research,lifescience,medical and 11 females, age at time of first sample [median (range)] 14 (10–16) years) were from patients aged less than 18 years and 56 samples (20 males, age at time of first sample [median Inhibitors,research,lifescience,medical (range)] 69 (65–87) years, and 36 females, age at time of first sample [median (range)] 68 (65–93) years) were from patients aged 65 years or greater. The results are summarized in Table 1 and in Figure 1. Samples per patient

were: 1 (304 patients), 2–5 (163 patients), 6–10 (15 patients), 11 or more (5 patients). Information on sample timing with respect to the last dose was available for 250 samples (26% of all samples received). For 229 Inhibitors,research,lifescience,medical samples the sampling time was greater than 7 hours postdose and with 21 samples up to 7 hours postdose. Where information was available 73% of males and 50% of females were smokers at the time of sampling (male Brefeldin_A smokers, 69; nonsmokers, 26; not recorded, 402; female smokers, 20; nonsmokers, 20; not recorded, 394). Table 1. Summary of quetiapine TDM data 2000–2011 (946 samples, 487 patients). Figure 1. Median (10th and 90th percentiles) plasma quetiapine concentrations (µg/l) by dose band. Number of samples in parentheses. Samples with (i) plasma quetiapine < 5 µg/l and (ii) adherence queried on the request form excluded. Information on coprescribed medication was available for 143 samples (15%).