Fluorescent chemosensors based on xanthenes

and related derivatives for the Hg2+ ions detection have been increasing due to the low cost and high applicability in industrial and biological processes [11]. During recent Volasertib mw years, novel inorganic-rhodamine hybrid sensors have been published. The rhodamine derivatives have been immobilized into the different inorganic receptors. Huang et al. reported fluorescent gold nanoparticle sensors for detection of Hg2+ ions [12]. Since gold nanoparticles (AuNPs) are highly efficient fluorescence quenchers, the rhodamine derivative had to be released from the AuNPs to restore the rhodamine fluorescence. Lee et al. and Zhou’s group developed a covalently bonded mesoporous silica rhodamine derivative [13, 14]. Childress and co-workers reported dye-doped polymer nanoparticles that are able to detect mercury ions. The nanoparticles were prepared by precipitation of highly fluorescent conjugated polymers and doped with rhodamine derivatives [15]. Recently,

Wang and Gao designed a mercury sensor using β-NaYF4:Yb3+/Eu3+ nanorods as the excitation source and a rhodamine derivative as a probe [16]. In this proposal, our research group has designed a new functional rhodamine derivative (Rh-UTES) that acts as a receptor of heavy metal ions. The Rh-UTES derivative was covalently bonded to porous silicon microcavity (PSiMc) to develop a hybrid sensor. The main advantage of the proposed method is the simplicity of the system and the fact that the hybrid sensor should be easy to carry for field applications. The PSiMc has proven p53 activator to be a suitable material with

unique optical properties for Cyclooxygenase (COX) the development of this kind of fluorescent sensor [17]. Our previous approaches in this field have shown that the detection of fluorescent molecules is possible using the optical properties of specific PSi structure (mirror or microcavity) [18]. Increased excitation and enhanced emission, both driven by the efficient reflection of light and resonance effects within the PSi microcavities, allowed the enhancement of the fluorescent response of the Rh-UTES derivative even at low molecular concentration. Hence, the variation of this method was used here to produce detection of low concentrations of heavy metals by forming metallic complexes within the pores that turn on the luminescence emission. Methods Rhodamine base, ethylenediamine, m-xylenediisocyanate, 3-aminopropyltriethoxysilane (APTES), hydrochloric acid, hydrofluoric acid, nitric acid, sodium hydroxide, and mercury nitrate were purchased from Sigma-Aldrich (St. Louis, MO, USA). All solvents were analytical reagent grade and used as received. Instruments and spectroscopy measurements The reflectivity spectra were recorded in an Agilent Cary 60 SB-715992 clinical trial UV-Vis spectrophotometer (Agilent Technologies, Sta.