Tunicamycin was used to establish the damage model in L02 cells. Methyl thiazolyl tetrazolium(MTT) colorimetric assay ended up being used to research the survival price of ethyl acetate plant from B. bipinnata in L02 cells injury induced by endoplasmic reticulum anxiety; the necessary protein expressions of endoplasmic reticulum stress-related molecule glucose regulated protein 78(GRP78), PKR-like ER kinase(PERK), eukaryotic initiation factor-2(eIF2α), activating transcription factor 4(ATF4), C/EBP homologous protein(CHOP), B-cell CLL/lymphoma 2(Bcl-2), Bal-2 associated X apoptosis regulator(Bax) had been analyzed by Wes-tern blot. The expressions regarding the above proteins had been also detected after endoplasmic reticulum stress inhibitor(4-phenyl butyric acid) and CHOP shRNA-mediated knockdowns were added. The expressions of GRP78, PERK, CHOP in L02 cells were seen by immunofluorchanism is related to the inhibition of endoplasmic reticulum stress as well as the down-regulation of apoptosis in cells through the PERK/eIF2α/ATF4/CHOP signaling pathway.This research founded superior liquid chromatography(HPLC) fingerprints of Chinese drugs derived from Apocynum venetum and Poacynum pictum in Xinjiang and explored their structure variations with all the mixture of material determination, similarity analysis, cluster analysis and principal component analysis. The HPLC conditions included Phenomenex Kinetex C_(18) column(4.6 mm ×100 mm, 2.6 μm), acetonitrile-0.01% trifluoroacetic acid aqueous solution as cellular stage, gradient elution, movement rate of 0.6 mL·min~(-1), detection wavelength of 281 nm and line heat of 25 ℃. The information of chlorogenic acid, quercetin-3-O-sophoroside, rutin, hyperin, isoquercitrin, trifolin and astragalin ended up being determined in 31 batches of medicinal products, and fingerprint analysis and chemometric evaluation had been Validation bioassay done with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004 A) and SPSS 21.0. Within the Chinese Pharmacopoeia 2020, the grade of Apocyni Veneti pictum from different habitats plus the selection of introduction and cultivation areas.Twenty-six compounds, including sixteen meroterpenoids(1-16), a triterpenoid(17), four terpenoid derivatives(18-21), and five aromatic compounds(22-26), were separated through the leaves of Psidium guajava. Their structures were identified by spectroscopic analyses including NMR and MS. Substances 21-26 were gotten from plants of Psidium the very first time. Based on the framework,(R)-2-ethylhexyl 2H-1,2,3-triazole-4-carboxylate(24 a), an α-glucosidase inhibitor recently separated from Paramignya trimera, should be revised as chemical 24. Meroterpenoids 1-16 were evaluated with regards to their antitumor and antifungal activities. Meroterpenoids psiguajadial D(4), guapsidial A(5), 4,5-diepipsidial A(7), guadial A(14), and guadial B(15) revealed cytotoxicities against five personal tumor cell lines(HL-60, A-549, SMMC-7721, MCF-7, and SW-480), among which 5 had been the most effective with an IC_(50) of 3.21-9.94 μmol·L~(-1).Compounds(1-6) had been isolated and identified from 90per cent ethanol extract associated with the stems and leaves of Cassia occidentalis through column chromatography with silica gel, ODS, and Sephadex LH-20. These substances were recognized as 7-hydroxy-5-(3-hydroxy-2-oxopropyl)-2-methyl-4H-chromen-4-one(1), saccharonol A(2), S-6-hydroxymullein(3), 2-methyl-5-acetonyl-7-hydroxy-chromone(4), 2-(2′-hydroxypropyl)-5-methyl-7-hydroxychromone(5) and 7,4′-dihydroxyflavone(6) according to their particular physicochemical and spectroscopic data. One of them, compound SN-001 purchase 1 was a new mixture, and all the compounds had been isolated using this plant for the first time. DPPH technique had been employed to determine the anti-oxidant tasks of the substances in vitro. Six substances exhibited weak anti-oxidant activities.Fifteen compounds(1-15) were isolated through the 95per cent EtOH plant for the entire herb of Physalis minima by numerous chromatography methods including silica solution, Sephadex LH-20, middle chromatogram isolated gel(MCI), octadecyl silica(ODS), and semi-preparative high performance liquid chromatography(HPLC). Their frameworks were elucidated by infrared spectroscopy(IR), ultraviolet spectroscopy(UV), high-resolution electrospray ionization mass spectrometry(HR-ESI-MS), atomic magnetic re-sonance(NMR), and circular dichroism(CD) as(5S)-5,11-dihydroxy-3-methyl-5-pentylfuran-2(5H)-one(1), withaphysalin R(2), withaphysalin Q(3), withaphysanolide A(4), phaseic acid(5), grasshopper ketone(6), 3S,5R-dihydroxy-6S,7-megastigmadien-9-one(7), vanillic acid(8), 2-trans,4-trans-abscisic acid(9), capillasterolide(10), 5,3′-dihydroxy-3,7,4′-trimethoxyflavone(11),(-)-loliolide(12), 4-hydroxyacetophenone(13), acetosyringone(14), and aurantiamide acetate(15). Compound 1 was a fresh butenolide, and compounds 5-7 and 10-12 had been isolated from the Physalis for the first time. Compounds 4, 13, and 15 were isolated the very first time from P. minima. Moreover, their anti-inflammatory task ended up being examined in vitro. Compound 12 was discovered to possess an inhibitory effect on the transcription of an NF-κB-dependent reporter gene in LPS-induced 293 T/NF-κB-luc cells at 10 μmol·L~(-1), showing an inhibitory price of 62.31%±4.8%.This study explored the chemical constituents of the aerial part of Hypericum curvisepalum. Sixteen substances had been isolated through the 95per cent ethanol herb of H. curvisepalum with numerous chromatographic practices, including a unique prenylated phenyl polyketide, mysorenone D(1). Various other compounds had been mysorenone-A(2), mysorenone-C(3), mysorenone-B(4), peplidiforone A(5), 4-methoxy-3-(2-methylbut-3-en-2-yl)-6-phenyl-2H-pyran-2-one(6), hyperenone-A(7), 4-(3,3-dimethylallyl)oxy-6-phenyl-α-pyrone(8), peplidiforone B(9), elegaphenone(10), hypercohin A(11), hyperisampsin G(12), spathulenol(13), quercetin(14), β-sitosterol(15), and β-amyrin(16).Fifteen bibenzyls had been separated and purified through the ethyl acetate plant for the stems of Dendrobium officinale by macroporous resin, MCI, silica solution, Sephadex LH-20, and ODS line chromatographies, as well as preparative thin-layer chromatography and preparative HPLC. The frameworks of substances were identified based on the spectra information of ~1H-NMR, ~(13)C-NMR, and MS, and also the physical and physiochemical properties dendrocandin X(1), 3,4′-dihydroxy-4,5-dimethoxybibenzyl(2), 6″-de-O-methyldendrofindlaphenol A(3), 3,4-dihydroxy-4′,5-dimethoxybibenzyl(4), dendrosinen B(5), 3,4,4′-trihydroxy-5-methoxybibenzyl(6), 3,3′-dihydroxy-4,5-dimethoxybibenzyl(7), 3,4′-dihydroxy-5-methoxybibenzyl(8), moscatilin(9), gigantol(10), 4,4′-dihydroxy-3,5-dimethoxybibenzyl(11), 3,4′,5-trihydroxy-3′-methoxybibenzyl(12), 3-O-methylgigantol(13), dendrocandin U(14), and dendrocandin N(15). Substance Optogenetic stimulation 1 was a novel compound. Compound 2 ended up being isolated from Dendrobium species the very first time.