Staining Analysis Immunoreactivity for Cx26 was considered to be positive if distinct staining of the cytoplasm was observed in at least 10% of the tumor cells (Fig. 1) and P53 was considered to be positive if distinct staining of the nuclei was observed Selleckchem Nutlin3a in at least 50% of tumor cells (Fig. 2). The apoptotic index (AI) was expressed as the number of apoptotic tumor cells divided by the total number of tumor cells in the same field with evaluation of 1000 nuclei in randomly selected areas in each specimen (Fig. 3). Figure 1 Immunohistochemical staing for Cx26 in colorectal cancer. Cytoplasmic Cx26 VX-680 manufacturer expression was found (×200). Figure 2 Immunohistochemical staing for P53 in colorectal cancer. Nuclear

P53 expression was found in most tumor cells (×200). Figure 3 Apoptotic index (AI) as evaluated by TUNEL (×200). The slides were examined by two independent pathologists who were not aware of the corresponding clinicopathological data. Any cases with discordant scores were reevaluated learn more a second time until a consensus was reached, no discrepancies between the evaluations were detected by the two

investigators. Statistical Analysis The data were compiled and analyzed using the SPSS software package for Windows (version 11.0; SPSS Inc., Chicago, Ill., USA). The relationship between Cx26 expression and the clinicopathological data, P53 and AI was evaluated by the chi-square test and Mann-Whitney U test. The disease specific survival was calculated by the Kaplan-Meier method and analyzed by the log-rank test. Prognostic factors were examined by univariate and multivariate analyses using a Cox proportional hazards model. P < 0.05 was considered to be significant. Results Cx26 expression was mainly localized in the cytoplasm of the cancer cells. In a few cases, we observed weak cytoplasmic staining in the normal mucosa. However

we did not consider this to be specific staining. Eighty-three of the 153 tumors (54.2%) showed Cx26 expression. Liothyronine Sodium P53 expression was observed in 71 (46.4%). The correlation between Cx26 and the clinicopathological features is summarized in Table 1. Cx26 expression had a statistically significant relationship with disease recurrence and the histological type (P < 0.05). Moreover P53 expression had a statistically significant relationship with Cx26 expression (P < 0.05). The disease specific survival according to the status of Cx26 expression is shown in Fig.4. The patients with Cx26 negative tumors had significantly worse survival than those with positive tumors (P < 0.05). Cx26 expression was an independent prognostic factor, as well as lymph node metastasis, blood vessels invasion according to a multivariate analysis (Table 2). There was no significant correlation between Cx26 and AI (Fig. 5). Table 1 Correlation between the Cx26 expression and clinicopathological features   Cx26     Negative Positive P-value Age (mean ± SD, years. 66.4 ± 8.1 66.4 ± 10.5   Gender       Male 41 46   Female 29 37 0.