After 5 h of administration, β-LG could not be detected in the PC group, suggesting that β-LG clearance required at least 5 h to occur. In the Bov group, low concentrations of β-LG (1.08 mg ml-1) were detected in animal sera after 5 h of β-LG administration (Figure 2). Figure 2 Concentration of β-lactoglobulin in animal sera from treatment groups. Upon an intragastrically dose of β-LG, blood was buy BAY 73-4506 collected at the indicated time points and the levels of β-LG in mice sera were determined by FPLC. buy GSK1210151A The results are shown as the average of β-LG concentration detected in a pool of animal’s sera from each experimental group (N = 8 mice per group), in two independent experiments.
(NC) negative control group; (Bov) mice treated with bovicin HC5; (PC) positive control group. Oral administration of bovicin HC5 and ovalbumin induce histological and morphometric alterations in the intestine of BALB/c mice No alterations were identified in the liver and heart of animals from all the groups analyzed (data not shown). A significant decrease in the total number of spleen cells was observed in Bov and PC groups, when compared to the NC group (Figure 3). Figure 3 Comparison of the total number of splenocytes among experimental groups. Data are shown as average
± SD, from two independent experiments (N = 8 mice per group). Statistically significant differences among treatments by the Dunn’s {Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|buy Anti-diabetic Compound Library|Anti-diabetic Compound Library ic50|Anti-diabetic Compound Library price|Anti-diabetic Compound Library cost|Anti-diabetic Compound Library solubility dmso|Anti-diabetic Compound Library purchase|Anti-diabetic Compound Library manufacturer|Anti-diabetic Compound Library research buy|Anti-diabetic Compound Library order|Anti-diabetic Compound Library mouse|Anti-diabetic Compound Library chemical structure|Anti-diabetic Compound Library mw|Anti-diabetic Compound Library molecular weight|Anti-diabetic Compound Library datasheet|Anti-diabetic Compound Library supplier|Anti-diabetic Compound Library in vitro|Anti-diabetic Compound Library cell line|Anti-diabetic Compound Library concentration|Anti-diabetic Compound Library nmr|Anti-diabetic Compound Library in vivo|Anti-diabetic Compound Library clinical trial|Anti-diabetic Compound Library cell assay|Anti-diabetic Compound Library screening|Anti-diabetic Compound Library high throughput|buy Antidiabetic Compound Library|Antidiabetic Compound Library ic50|Antidiabetic Compound Library price|Antidiabetic Compound Library cost|Antidiabetic Compound Library solubility dmso|Antidiabetic Compound Library purchase|Antidiabetic Compound Library manufacturer|Antidiabetic Compound Library research buy|Antidiabetic Compound Library order|Antidiabetic Compound Library chemical structure|Antidiabetic Compound Library datasheet|Antidiabetic Compound Library supplier|Antidiabetic Compound Library in vitro|Antidiabetic Compound Library cell line|Antidiabetic Compound Library concentration|Antidiabetic Compound Library clinical trial|Antidiabetic Compound Library cell assay|Antidiabetic Compound Library screening|Antidiabetic Compound Library high throughput|Anti-diabetic Compound high throughput screening| multiple comparison test (p < 0.05) were indicated by different lowercase letters (“a” or “b”) above the error bars. (NC) negative control group; (Bov) mice treated with bovicin HC5; (PC) positive control group. The small intestine of the NC group presented a well-preserved villi and crypts, with intact intestinal layers (Figure 4A and 4D). In the Bov group, the severity of the effects varied among the animals and major alterations were observed
in the lamina propria (mild edema) and in the apical portion of the villi, with a “worst case scenario” being presented in Figure 4B and 4E. As expected, Diflunisal the animals from the PC group developed intestinal inflammation, characterized by inflammatory cell infiltration, tissue destruction, epithelial exulceration, edema and congestion of the lamina propria (Figure 4C and 4F). Figure 4 Photomicrographs of longitudinal sections of small intestine of the experimental groups. Jejunum segments were collected and processed for optical microscopy analysis at the end of the experiment (day 58) (N = 8 mice per group). (NC), negative control group, figures A and D; (Bov) mice treated with bovicin HC5, figures B and E; (PC) positive control group, figures C and F. The sections were stained with hematoxylin and eosin (HE; left panel) or PAS/Alcian Blue (right panel). Abbreviations: L: lumen; EP: simple cuboidal epithelium; BB: brush border; V: villum; LP: lamina propria; LC: Lieberkühn crypt; Sm: submucosa; IC: inner circular muscle layer; OL: outer longitudinal muscle layer.