Analysis of the early Lenalidomide stage HLB response subnetwork At early stage, the HLB bacterium could rarely be detected, nor any HLB symptom observed, but the re sponse to HLB in citrus could occur early at least at the transcriptional level. Therefore, we decided to analyze the subnetwork for the early stage HLB re sponsive genes. A total of 222 Probesets, including 158 up regulated and 62 down regulated Probesets, were used as the seed nodes to map the HLB response network, resulting in the HLB early response subnetwork. This subnetwork based on the first degree neighbors of these seed nodes contains 461 Probesets and 683 interactions. Inhibitors,Modulators,Libraries Among those Probesets, 29 are involved in carbohydrate metabolic process, 23 in nitrogen and amino acid metabolic process, 67 in transport, 27 in defense response, 24 in signaling and 24 in hormone re sponse.

GO enrichment analysis shows that carbohydrate Inhibitors,Modulators,Libraries metabolic process, transport and defense are overrepre sented. Although the hormone response category is not overrepresented, JA response consisting 10 Probesets is overrepresented with a p value of 0. 01. Therefore, our analysis of the early stage subnetwork indicates that even at Inhibitors,Modulators,Libraries this stage, several im portant biological processes have been activated or inacti vated. In the HLB early response subnetwork, there is only one subset that has several large hubs, while all other small subsets have interactions that are not con nected further. To provide further detail of the early stage response in citrus, we analyzed the two nodes in the large subset of this subnetwork, Cit. 29252. 1.

S1 s at, and Cit. 12214. 1. S1 s at. Cit. 29252. 1. S1 s at represents a triacylglycerol lipase gene most closely related to Arabidopsis EDS1. Extracting this EDS1 like gene from the HLB early response subnetwork shows that EDS1 interacts with 15 Probesets. Inhibitors,Modulators,Libraries Among these Probesets, one Probeset Inhibitors,Modulators,Libraries has interactions with only two other Probesets, five Probesets form the large hubs each with 50 113 interactions, and nine other Probesets form the medium size hubs with 11 44 interactions. The fact that Cit. 29252. 1. S1 at connects with the five large hubs indi cates a potentially critical role in citrus response to the HLB bacterial infection. Cit. 6535. 1. S1 at represents a carbohydrate transmembrane transporter or phosphate transmembrane transporter, Cit. 10234. 1.

S1 s at is closely related to CB5 E involved in heme binding, Cit. 4135. 1. S1 s at represents a putative CC NBS LRR class disease resistance protein, and Cit. 2933. 1. S1 s at is very similar to Arabidopsis HMGB1 involved in transcriptional control through chromatin selleckchem Rapamycin remodeling. In addition, some of the medium size hubs that interact with the EDS1 like gene play important roles in protein modifications or lipid me tabolism. For example, Cit. 39054. 1.