Consistent with this value, the mean diameter of empty emulsomes was pre viously Sunitinib buy reported to be 297 28 nm. In addition, zeta potential of CurcuEmulsomes is comparable to that of empty emulsomes. With the aid of the auto fluorescence properties of curcu min, it was possible to evidence the incorporation of curcumin into emulsomes and that the prepared nano carrier system is a stable dispersed formulation in water. Consequently, the presented data declines any significant influence of incorporated drug neither on the size nor on the surface potential of the nanocarrier, which could further affect the particular dispersity of the nanocarrier in water. Inhibitors,Modulators,Libraries Tautomeric curcumin incorporated into CurcuEmulsomes in its enol form Curcumin is a yellow colored tautomeric compound that, upon dissolution in an organic solvent, absorbs light in the visible wavelength range.
In nonpolar, i. e. aprotic sol vents such as chloroform, the spectrum displays vibronic structure with Inhibitors,Modulators,Libraries max near 420 nm. This feature corresponds to Inhibitors,Modulators,Libraries the fully conjugated form of the protonated enol. In polar protic solvents such as DMSO, the vibronic features are no longer resolved, and hence, the molar absorptivity decreases as solvent polarity increases resulting in max Inhibitors,Modulators,Libraries shifts to nearly 430 nm. In agreement with this, the UV vis spectrum of CurcuEmulsomes dis played the same max as curcumin in chloroform, and differed from max of curcumin dissolved in DMSO. Hence, curcumin incorporated in CurcuEmul somes is evidently in its fully conjugated protonated enol form.
Like the absorbance spectrum, the emission spectrum of CurcuEmulsomes pursued that of curcumin in chloroform and showed a max at 500 nm. Excitated at 420 nm, free curcumin in DMSO showed an emission peak centered at 520 nm and curcumin in water did not fluoresce. Curcumin composition inside CurcuEmulsomes Since turmeric as a mixture was Inhibitors,Modulators,Libraries demonstrated to have the same inhibitory effect as pure curcumin, curcu min was used as purchased without any further purifica tion. Therefore, the turmeric fed to the system contained all three analogues, i. e. curcumin, DMC and BDMC. HPLC analysis showed that the turmeric extract consisted of 78. 1% curcumin, 17. 7% DMC and 4. 1% BDMC, whereas CurcuEmulsomes comprised of 40. 8% curcumin, 40. 3% DMC and 16. 8% BDMC. As curcumin analogues were the only substances in Cur cuEmulsomes raising a peak at 420 nm, empty emulsomes did not show any peak in HPLC analysis.
Effect of CurcuEmulsomes on HepG2 cell viability Previous studies demonstrated that 10 50 uM curcumin induces cell death primarily through apoptosis. Within this range, HepG2 cells were treated with CurcuEmulsomes and free curcumin of the same concentrations, respectively. After treatment for 6, 24 and 48 hours, the cell viability was determined with CellTiter sellekchem Blue assay.