Regardless of the mechanism, higher bacterial MP under MRG conditions may contribute towards increased survival under the conditions examined. Another important cellular property examined in this study is membrane integrity (MI). Like MP, higher MI is strongly correlated with bacterial viability [61]. Higher MI was found under MRG conditions for both E. coli and S. aureus

grown in LB, but not in M9 minimal media and diluted LB, respectively. Dramatically https://www.selleckchem.com/products/Trichostatin-A.html higher percentages of dead cells were found under normal gravity conditions in rich media. Interestingly, in congruence with earlier E. coli gene expression studies [33], MP and MI observations are consistent with the observation that E. coli grown under MRG conditions exhibits enhanced ability to survive

sub-lethal doses of antimicrobial agents [13, 22]. As these stress- survival assays require growth Selonsertib nmr of E. coli in culture, it is possible that differences in MP and MI account for bacterial phenotypes observed under MRG conditions. Conclusions Documented responses to MRG or microgravity conditions include large scale changes in gene expression as well as more basic responses, such as higher cell numbers. Our study demonstrates that such changes are accompanied by increased membrane potential and lower percentages of dead cells both of which are critical to bacterial population growth. The two species examined, generally, exhibited similar responses. However, responses observed varied with growth phase and were medium-dependent revealing that nutrient availability is a modulator of responses to these conditions. Overall, our data provides novel information about E. coli and S. aureus MP and MI under MRG conditions and suggest that bacteria are physiologically more active and a larger percentage

are viable under MRG as compared to NG conditions. Future studies are needed to elucidate the mechanism leading to increased MP and MI and to determine if these differences are consistently observed regardless of bacterial species and growth conditions. Finally, our findings have LCZ696 mw implications for fundamental biological next responses, namely the ability for living cells to detect and respond to mechanical stimuli [19]. Further study is needed to examine the inter-play between responses to mechanical conditions and other aspects of the environment and to explore potential mechanisms by which such conditions are sensed or detected to determine if they are conserved across taxa. Methods Bacterial strains Escherichia coli K-12 MG1655 (ATCC 700926), Staphylococcus aureus (ATCC 25923) Growth media Full strength Luria broth (LB) and M9 Minimal media (+ 0.4% glucose and 1 μg/ml thiamine) were used to cultivate E. coli. Full strength LB and diluted LB (1:50) were used to cultivate S. aureus. In this case, diluted LB was used instead of M9 minimal media because M9 did not support the growth of S. aureus (data not shown).