Effect OF ANGIOGENESIS INHIBITOR CILENGITIDE ON GLIOBLASTOMA Development IN NUDE MICE Shinya Yamada,1,5 Xing Yao Bu,two Vazgen Khankaldyyan,2 Stefan Bluml,3,5 Ignacio Gonzales Gomez,four J. Gordon McComb,one,5 Anat Erdreich Epstein,two and Walter E. Laug2, Departments of 1Neurological Surgical treatment, 2Pediatrics, 3Radiology and 4Pathology, Keck School of Medication, University of Southern California, Los Angeles and Childrens Hospital Los Angeles, Los Angeles, CA, USA, and 5The Rudi Schulte Study Institute, Santa Barbara, CA, USA The aim of this study was to find out the effect in the angio genesis selleck chemical inhibitor Cilengitide on glioblastoma growth and connected angiogenesis within the brains of nude mice. U87MG human glio blastoma cells in one ML medium had been stereotactically injected above twenty min in to the caudate/putamen of nude mice. Mice have been injected every day IP with Cilengitide or solvent beginning five days soon after tumor implan tation.
Mice have been killed 1 hr to 63 days after tumor implantation and examined for tumor dimension, vascularity, apoptosis, and tumor cell prolifera tion. This injection system resulted in very reproducible, TW37 localized, spherical tumor cell placement while in the parenchyma, not having reflux into the subarachnoid room or penetration in to the ventricle. Serial brain sections showed steady tumor size for your 1st 30 40 days. Thereafter, manage tumors showed exponential development to a maximal volume of 120 mm3, leading to signs of distress that essential us to kill the mice at 8 9 weeks. Serial staining for Ki 67, a marker for cell proliferation and CD31, an endothelial cell marker, demonstrated that tumor cell proliferation and tumor angiogenesis elevated a lot more than the improve in tumor volume. In contrast, in Cilengitide taken care of mice, the tumor volume remained secure at 1 2 mm3, and staining for Ki 67 and CD31 remained lower all through the 9 weeks.
This standardized brain tumor model is highly reproducible and valuable for testing new treatment method regimens. Cilengitide is highly successful at suppressing blood vessel growth and tumor cell proliferation, thereby controlling the orthotopic development of this glioblastoma cell line. It stays to be established whether the impact of Cilengitide is due to a mixture of inhibition http://t.co/MfAIst4oCe

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of angiogenesis and direct inhibition of tumor cell prolifera tion or only direct inhibition of angiogenesis with secondary inhibition of tumor cell proliferation. ET 40. BLOCKING CXCR4 MEDIATED cAMP SUPPRESSION INHIBITS BRAIN TUMOR Growth IN VIVO Lihua Yang,1 Erin Jackson,two B. Mark Woerner,one Arie Perry,3 David Piwnica Worms,two,four and Joshua B. Rubin1,5,6, Departments of 1Pediatrics, two Molecular Imaging Center, Mallinckrodt Institute of Radiology, 3 Pathology, 4Molecular Biology and Pharmacology, 5Anatomy and Neurobiology, 6Neurology, Washington University School of Medication and St. ET 39.