The patient was successfully

treated with debridement of his tongue and left carotid endarterectomy. (J Vasc Surg 2011;54:837-9.)”
“The system described here allows the expression of protein fragments into a solvent-exposed loop of a carrier protein, the beta-lactamase BlaP. When using Escherichia coli constitutive expression vectors, a positive selection of antibioresistant bacteria expressing functional hybrid beta-lactamases is achieved in the presence of beta-lactams making further screening of correctly folded and secreted hybrid RAD001 research buy beta-lactamases easier. Protease-specific recognition sites have been engineered on both sides of the beta-lactamase permissive loop in order to cleave off the exogenous protein fragment from the carrier protein by an original two-step procedure. According to our data, this approach constitutes a suitable alternative

for production of difficult to express protein domains. This work demonstrates that the use of BlaP as a carrier protein Napabucasin mouse does not alter the biochemical activity and the native disulphide bridge formation of the inserted chitin binding domain of the human macrophage chitotriosidase. We also report that the beta-lactamase activity of the hybrid protein can be used to monitor interactions between the inserted protein fragments and its ligands and to screen neutralizing molecules.”
“Introduction: Up-regulation of the type 2 cannabinoid receptor (CB2R) has been reported in (neuro)inflammatory diseases. AZD3965 In this study, we report the preclinical evaluation of [C-11]NE40 as positron emission tomography (PET) radioligand for visualization of the CB2R.

Methods: The selectivity

of NE40 for CB2R and its toxicity and mutagenicity were determined. [C-11]NE40 was evaluated by biodistribution and autoradiography studies in normal rats and a microPET study in normal mice, rats and a rhesus monkey. Specific in vivo binding of [C-11]NE40 to human CB2R (hCB(2)R) was studied in a rat model with hCB(2)R overexpression.

Results: [C-11]NE40 shows specific CB2R binding in the spleen and blood of normal rats and high brain uptake in rhesus monkey. [C-11]NE40 showed specific and reversible binding to hCB(2)R in vivo in a rat model with local hCB(2)R overexpression.

Conclusions: [C-11]NE40 shows favorable characteristics as radioligand for in vivo visualization of the CB2R and is a promising candidate for hCB(2)R PET imaging. (C) 2012 Elsevier Inc. All rights reserved.”
“We report the first documented case of distal thromboembolism originating from an abdominal aortic aneurysm (AAA) after a blunt trauma. A 72-year-old man with a known 6.2 cm AAA was brought to our emergency department with signs of bilateral acute limb ischemia developing immediately after an accidental fall.