To test this hypothesis, we designed an assay to fol examined in

To test this hypothesis, we designed an assay to fol examined in E. tenella is upregulated in merozoites fur ther underscores Inhibitors,Modulators,Libraries the importance of proteases in the biol ogy of the asexual stages of apicomplexan parasites. Not surprisingly, therefore, an eimepsin, several cathepsins, a calpain, a trypsin like protease, subtilisins, Clp and a rhomboid protease are upregulated in the asexual stages of E. tenella. Likewise, eimepsin1 and insulysin 3 are expressed specifically in oocysts and may play an important role in the first steps of the parasite lifecycle, such as host cell invasion, they are, therefore, worthy of further research. The downregulation of several pro teases in sporu lated oocysts may be, in part, attributed to the dormancy of this lifecycle stage, yet still warrants further investigation.

Perhaps the most significant finding of Inhibitors,Modulators,Libraries our stage specific expression study was the relatively large number of protease genes whose expression is upregulated spe cifically in the gametocytes stage a total of at least 13 genes, including six that are only expressed in gameto Inhibitors,Modulators,Libraries cyte. This observation becomes even more intriguing when examined in the context of the low the degradation of GAM56 in freshly harvested gametocytes. This assay has certain inherent limitations, first, it relies on sensitive antibodies for de tection of specific degradation of GAM56 and, unfortu nately, the lack of suitable antibodies for detection of GAM82 in E. tenella meant that we were unable to run confirmatory experiments with this protein, and, second, the only controls possible are a zero time point and a cocktail of protease inhibitors designed to prevent all proteolytic activity.

These limitations require us to be cautious in our Inhibitors,Modulators,Libraries interpretations, none the less, the inhib ition of degradation of native GAM56 by a very specific group of protease inhibitors reveals that this function may be carried out by subtilisin like proteases. Thus, degradation of GAM56 was inhibited by the serine cyst eine protease inhibitors, chymostatin and leupeptin, and the serine protease specific inhibitors, benzamidine HCL, soybean trypsin inhibitor and aprotinin but not by AEBSF. Intriguingly, the metal chelating agent, Inhibitors,Modulators,Libraries EDTA, also inhibited degradation of GAM56. This profile indicates that serine proteases EPZ-5676 Histone Methyltransferase inhibitor are critical for degradation of GAM56 but it seems to rule out participation of rhomboid pro teases, which are unaffected by EDTA, aprotonin, leupeptin and chymostatin.

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