We then considered different theoretical distributions for foci between slices if excluded from increasing percentages (with 10% steps) of the cell periphery and/or the cell centre by subtracting circle areas (examples are shown in Figure 2, 3 and 4). Observed distributions
were compared to calculated distributions using the χ2 test http://www.graphpad.com/quickcalcs. Distributions were considered to be different if the associated p-values were less than 0.05. Pearson’s buy STA-9090 correlation coefficients between cell length and cell width distributions were calculated using Excel software. Acknowledgements We thank Thierry Enjalbert for preliminary constructs, and O. Espeli for the gift of plasmids and strains. We thank Roland Barriot and Hervé Seitz for help with the statistics, Philippe Guynet for help with mathematics; and Christian Lesterlin and Suckjoon Jun for helpful discussions. This work was funded by internal funding from the CNRS and University of Toulouse and by a grant from the Agence Nationale de la Recherche (ANR contract BLAN06-2 134012). https://www.selleckchem.com/products/nu7441.html Electronic
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