40% GC content (Figure 5 and Table 3) Of the 3,163 predicted gen

40% GC content (Figure 5 and Table 3). Of the 3,163 predicted genes, 3,113 were protein-coding genes, and 50 were RNAs. A total of 1,977 genes (62.50%) no were assigned a putative function. Eighty-one genes were identified as ORFans (2.6%). The remaining genes were annotated as hypothetical proteins. The properties and statistics of the genome are summarized in Tables 3 and distribution of genes into COG functional categories is presented in Table 4. Figure 5 Graphical circular map of the chromosome. From outside to the center: Genes on the forward strand (colored by COG categories), genes on the reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red), GC content, and GC skew.

Table 3 Nucleotide content and gene count levels of the genome Table 4 Number of genes associated with the 25 general COG functional categories Comparison with the genomes from other Alistipes species To date, the genome from Alistipes shahii strain WAL 8301 is the only genome from the Alistipes genus that has been sequenced. By comparison with A. shahaii, A. senegalensis exhibited a higher G+C content (57.2% vs 58.40%, respectively), a higher number of genes (2,616 vs 3,163) and a smaller number of genes with peptide signal (989 vs 712). Moreover, A. senegalensis had higher ratios of genes per Mb (696 vs 788) and a comparable number of genes assigned to COGs (58.9 vs 59.3). However, the distribution of genes into COG categories (Table 4) was highly similar in both genomes. In addition, A. senegalensis and A. shahaii shared a mean 89.9% (range 78.4-100%) sequence similarity at the genome level.

Conclusion On the basis of phenotypic, phylogenetic and genomic analyses, we formally propose the creation of Alistipes senegalensis sp. nov. that contains the strain JC50T. This bacterium has been found in Senegal. Description of Alistipes senegalensis sp. nov. Alistipes senegalensis (se.ne.gal.e��n.sis. L. gen. masc. n. senegalensis, of Senegal, the country of origin of Alistipes senegalensis). Colonies are 0.2 to 0.3 mm in diameter on blood-enriched Columbia agar and Brain Heart Infusion (BHI) agar. Cells are rod-shaped with a mean diameter of 0.56 ��m. Optimal growth is achieved anaerobically. Weak growth is observed in microaerophilic conditions. No growth is observed in aerobic conditions. Growth occurred between 30-37��C, with optimal growth observed at 37��C, in BHI medium + 5% NaCl.

Cells stain Gram negative and are non-motile. Catalase, ��-galactosidase, ��-galactosidase, ��-glucuronidase, arginine arlyamidase, glycine arylamidase, Dacomitinib proline arylimidase, leucyl glycine arylamidase, and alanine arylamidase activities are present. Mannose fermentation and indole production are also present. Oxidase activity is absent. Cells are susceptible to penicillin G, amoxicillin + clavulanic acid, imipeneme and clindamycin but resistant to metronidazole. The G+C content of the genome is 58.40%.

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