Classification As the 16S rRNA gene analysis (Figure 1) indicated intermixed positions of Phaeobacter and Leisingera species (even though with low bootstrap support), selleck kinase inhibitor the classification of the group might need to be reconsidered. We thus conducted a preliminary phylogenomic analysis using GGDC [55-57] and the draft genomes of the type strains of the other Leisingera and Phaeobacter species. The results shown in Table 6 indicate that the DNA-DNA hybridization (DDH) similarities calculated in silico of P. articus to other Phaeobacter species are, on average, not higher than those to Leisingera species. The highest value is actually obtained for L. nanhaiensis and formula 2, which is preferred if genomes are only incompletely sequenced [55]. The overall low similarity values indicate that P.
arcticus might better be placed in a separate genus, particularly if compared to the according similarity values between the other Leisingera and Phaeobacter species [58,59]. Table 6 DDH similarities between P. arcticus DSM 23566T and the other Phaeobacter and Leisingera species (with genome-sequenced type strains) calculated in silico with the GGDC server version 2.0 [55]. The standard deviations indicate the inherent uncertainty in estimating DDH values from intergenomic distances based on models derived from empirical test data sets (which are always limited in size); see [57] for details. The distance formulas are explained in [55]. The numbers in parentheses are IMG object IDs (GenBank accession number in the case of P. gallaeciensis) identifying the underlying genome sequences.
Acknowledgements The authors would like to gratefully acknowledge the assistance of Iliana Schr?der for growing P. arcticus cultures and Evelyne-Marie Brambilla for DNA extraction and quality control (both at the DSMZ). The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under contract No. DE-AC02-05CH11231; the work conducted by the members of the Roseobacter consortium was supported by the German Research Foundation (DFG) Transregio-SFB 51.
Bacillus massilioanorexius strain AP8T (= CSUR P201 = DSM 26092) is the type strain of B. massilioanorexius sp. nov.
This bacterium is a Gram-positive, GSK-3 non-spore-forming, aerobic and motile bacillus that was isolated from the stool of a 21-year-old Caucasian French female suffering from a severe form of anorexia nervosa since the age of 12 years and is part of a ��culturomics�� study aiming at cultivating all species within human feces individually [1-3]. This bacterium was one of the 11 new bacterial species isolated from this single stool sample [3]. The current classification of Bacteria and Archaea remains a subject of debate and currently relies on a combination of phenotypic and genotypic characteristics [4]. Genomic data has not yet been routinely incorporated into descriptions.