62–1.25 mg/ml), as compared to that of the crude extract (MIC=1.25-5 mg/ml) or other fractions (MIC=1.25->5 mg/ml). However, none of the samples

tested was as active as the reference antibiotic, griseofulvin (MIC=0.001-0.020 mg/ml) (table 2). Table 1 The percentage inhibitions (%) of fungal growth by the crude extract of stem bark of Coula edulis and the fractions from the extract at the concentration of 5 mg/ml against the tested dermatophytes. Table 2 Minimum inhibitory concentration/minimum Inhibitors,research,lifescience,medical fungicidal concentration (MIC/MFC) (in mg/ml) of the crude extract of Coula edulis stem bark and fractions and compounds isolated from the extract against the tested dermatophytes. Acute Toxicity The results of the acute toxicity study indicated that female mice were more tolerant than male Inhibitors,research,lifescience,medical ones to oral AP24534 order administration of the crude extract. For doses up to 16 g/kg BW, the animal’s reaction to pinch and noise were reduced. All animals developed diarrhea within 3 hours after the administration of doses ≥16 g/kg BW. None of the treated mice survived

within 48 hours after the administration of 24 and 28 g/kg BW in males and females, Inhibitors,research,lifescience,medical respectively. The calculated LD50 values were 16.80 and 19.60 g/kg BW for male and female mice, respectively. Sub-acute Toxicity General Symptoms, Body and Organ Weight Changes No death did occur following Inhibitors,research,lifescience,medical daily administration of vehicle or the extract for 28 days in control or extract treated group, respectively. The animals did not show any significant changes in their general behaviur. However, there were significant dose-dependent decreases in animals’ body weight gain as well as food and water consumptions. (figure 2-​-3).3). These reductions were most pronounced (P<0.05) in the animals that were administered the extract at the highest dose (200

Inhibitors,research,lifescience,medical mg/kg BW). Moreover, the macroscopic observation of livers of animals revealed the presence of white vesicles on the surface of this organ at the dose of 200 mg/kg BW (figure 4). Also, significant decreases (P<0.05) were recorded in the relative liver, heart, lung and kidney weights at the same dose irrespective of the sex (figure 5). None of the screened organ showed significant (P≥0.05) variation in the parameters evaluated above at doses ≤100 mg/kg BW (figure 5). Figure 2 Variation below of relative body weight of rats as a function of the duration and dose of Coula edulis CH2Cl2/MeOH (1:1) stem bark extract. Figure 3 Daily amounts of food and water consumed as affected by doses of Coula edulis CH2Cl2/MeOH (1:1) stem bark extract during sub-acute toxicity study. Figure 4 Livers from rats treated with the CH2Cl2/MeOH (1:1) extract of Coula edulis stem bark (A) or vehicle (Dimethylsulfoxide, DMSO) (B). They can be differentiated by the presence of white vesicles on liver from rats receiving the extract.