Acetobacter diazotrophicus ), a promising diazotrophic endophyte in tropics. Curr Sci 2002, 83:137–145. 33. Tsuda K, Kosaka Y, Tsuge S, Kub Y, Horin O: Evaluation of the endophyte Enferobacfer cloacae SM10 isolated from spinach roots for biological control against fusarium wilt of spinach. J Gen Plant Pathol 2001, 67:78–84.CrossRef 34. Sambrook J, Fritsch EF, Maniatis T: Molecular cloning: a laboratory manual. 2nd edition. Cold Spring Harbor SNX-5422 Laboratory Press, Cold Spring Harbor, N Y; 1989.
35. Yoshida S, Hiradate S, Tsukamoto T, Hatakeda K, Shirata A: Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves. Phytopathol 2001, 91:181–187.CrossRef 36. Ramos HJO, Roncato-Maccari LDB, Souza EM, Soares-Ramos JRL, Hungria M, Pedrosa FO: Monitoring Azospirillum
-wheat interactions using the gfp and gusA genes selleck chemicals llc constitutively expressed from a new broad-host range vector. J Biotechnol 2002, 97:243–252.PubMedCrossRef 37. Schwyn B, Neilands JB: Universal chemical assay for the detection and determination of siderophores. Anal Biochem 1997, 160:46–56. 38. Gordon AS, Weber RP: Colorimetric estimation of indole acetic acid. Plant Physiol 1951, 26:192–195.PubMedCrossRef 39. Vazquez P, Holguin G, Puente ME, Lopez-Cortes A, Bashan Y: Phosphate-solubilizing AZD6738 cell line microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biol Fertil Soils 2000, 30:460–468.CrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions XL was responsible for designing the study, collected and prepared the tissues and contributed to write the manuscript. GB carried out antifungal activity analysis of Lu10-1 strain. YP carried out localization analysis of the strain. HJ and BY carried out plant growth-promoting analysis. LR and ZM were responsible for designing the study and contributed to write the manuscript. Myosin All authors edited the manuscript
and approved the final version.”
“Background M. tuberculosis is one of the most devastating human pathogens, and its threat to human health has intensified with the emergence of multidrug-resistant tuberculosis (TB) and the worldwide prevalence of co-infection with HIV [1, 2]. Two-component regulatory systems (TCRs) are widely distributed among bacteria and plants and enable organisms to regulate gene expression in response to a variety of environmental stimuli [3, 4]. Some TCRs are clearly involved in regulating the virulence of pathogenic bacteria [3]. The M. tuberculosis genome contains 11 paired TCRs and several orphan kinases and regulators [5]. Several TCRs are apparently required for the growth of M. tuberculosis under specific conditions [6–8]; for example, mprA-mprB is important for the maintenance of persistence [6]. Of the 11 M.