The structural changes indicate a reduction, or loss, of absorptive activity in this region of the larval body. Despite this, the secretory activity was maintained, which can be verified by the presence of electrondense granules and formation of secretory vesicles. The middle region of the sporocyst body, named membranous sac, is where the cercariae concentrated (Tang and Tang, 1977). Jang (1969) stated that when the sporocysts are expelled

the external wall detaches from the inner wall and the endocyst coils and occupies the swollen region. Details of these regions are also showed by Franco-Acuña et al. (2011) to daughter sporocysts of E. coelomaticum. Tang (1950) stated that when the swollen cyst is formed, the outer cysts wall apparently dies, once no further movement is noticed. The membranous sac region seen here shows a degenerated appearance, with degenerated see more membranes containing myelin figures. The lamellar structure of the membranous sac is probably important to reduce the water loss and dehydration

of the larva exposed to the environmental conditions after emergence. The muscle layer was degenerate and may be placed near or well away from the external surface of the membranous sac. Secretory and excretory activity was still occurring, once vesicles were formed in the tegument surface and the flame cell structure was maintained. The endocyst was cited by Tang (1950) as an inner cyst wall. By the first time it was possible to Dabrafenib cell line observe that the endocyst was not a membranous structure, but composed by a fibrilar material supported by a thick basal lamina. Moreover, inside the endocyst membranous structures amorphous material was observed. SPTLC1 These components are probably essential to prevent mechanical damage to the cercariae packed in the endocyst when exposed to the external conditions and ingested by the second intermediate host. According to Tang (1950)

the posterior region of the expelled sporocyst contracts pushing the cercariae to the middle of the larval body. Probably, when this region contracts the membranes coils and begins a degenerative process, leading to the myelin figures. The contractile ability and absorptive processes in the posterior region of sporocyst body seem to be maintained. Thus, the anterior and posterior regions of expelled daughter sporocyst did not die when exposed to the external environmental differently to E. pancreaticum as stated by Tang (1950). By the first time a detailed description of the ultrastructural organization of the mother and daughter sporocysts of E. coelomaticum and the changes that occurred through larval development were made. According to Ehlers (1985), new information on comparative morphology based on LM and electron microscopy provides valuable characteristics in elucidating phylogenetic relationships.