Sequences were analysed using the BlastX algorithm  compared to the protein sequence Crizotinib manufacturer database (GenBank). Growth measurement in presence of different concentrations of metal(loid)s The wild type see more strain C. testosteroni S44, iscR mutants C. testosteroni iscR-280, iscR-327 and iscR-513, and a mutant of iscR downstream, iscS + 30, were inoculated into 5 ml liquid LB medium supplemented with differing concentrations of Se(IV) encompassing 10.0, 25.0, 50.0 and 100.0 mM, respectively at 28°C with shaking at 180 rpm. Likewise, the wild type strain and four mutants were inoculated into 5 ml liquid LB medium supplemented with As(III), Cu(II) and Cd(II), respectively. The concentrations of As(III) were
0, 1.0, 5.0, 10.0, 20.0 mM, for Cd(II) they were 0, 0.1, 0.5, 1.0 mM, and for Cu(II) they were 0, 0.1, 1.0, 2.0, 4.0 mM, respectively. Cells were incubated at 26°C with shaking at 180 rpm. The OD600 value was determined after 24 h incubation. Acknowledgment We thank Prof. Dr. Klaus Qvortrup at CFIM of University of Copenhagen, and Dr. Takeshi Kasama and Wilhelmus Huyzer at Center for Electron Nanoscopy at the Technical University of Denmark for excellent work including bacterial sample preparation, TEM-EDX and EDS Mapping. We also thank Dr. Qin at the Electron Microscope Center of Huazhong Agricultural University. Funding This work was supported by the Natural Science Foundation of China (41171213),
China CSC Grant and by a fund of the Tobacco Company of Enshi, Hubei Province, P. R. China. Additional file Additional file 1: Figure S1. TEM graphs of C. testosteroni S44 amended with 1.0 mM Se(IV) at different times of incubation. LOXO-101 price B and D, strain S44 amended with Se(IV) at log phase and stationary phase, respectively. A and C are control (no Se(IV) ) at log phase and stationary phase, respectively. Arrows indicated extracellular selenium particles. References 1. Winkel LH, Johnson CA, Lenz M, Grundl T, Leupin OX, Amini M, Charlet L: Environmental selenium research: from microscopic processes to global understanding. Environ Sci Technol 2012, 46(2):571–579.PubMedCrossRef
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